Bio-Efficacy
In vitro scoring for screening anti cancer
compounds
Signal transduction networks play a key role
in the regulation of myriad essential cellular responses. The
tyrosine kinases are enzymes that have a central role in signal
transduction. EGFR-TK is one such transmembrane receptor tyrosine
kinase and its over expression has been detected in all stages of
tumor progression. The over expression may be the result of excess
ligand expression or high expression of EGFR, activating mutation,
failure of inactivation mechanisms, or transactivation through
receptor dimerization. Signalling through EGFR-TK is pleotropic both
in terms of the multiple signaling pathways that are activated and
in terms of the biologic downstream effects. Therefore, EGFR-TK is
an attractive drug target for inhibiting cancer. Set of synthetic
in-house compounds which are synthesized based on the classical data
with respect to EGFR are under the process of screening in in vitro
system.
As a first step towards the evaluation of the
cytotoxic potential of the proposed compounds, a screen which consists
of a panel of human cancer cell lines is used for various cell based
assays. The in vitro studies will be followed by in vivo studies on
tumor induced rats.
